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cell signaling technology 349 2933t  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc cell signaling technology 349 2933t
    Cell Signaling Technology 349 2933t, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 663 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+signaling+technology+349+2933t/Bim+Rabbit+mAb/10__1016_slash_j__cellin__2026__100323-165-122-122
    Average 96 stars, based on 663 article reviews
    cell signaling technology 349 2933t - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: Capicua mediates interleukin-2 withdrawal–induced death of activated CD8+ T cells
    Article Snippet: .. Materials and methods 336 4.1 Reagents and antibodies 337 Reagents and antibodies used in this study were obtained from the indicated sources: 338 Recombinant human IL-2 (SL Pharm, S19991010); Annexin V-FITC/PI Apoptosis Kit 339 (Elabscience, E-CK-A211); Actinomycin D (MCE, HY-17559 ); TrueCut Cas9 340 Protein (Invitrogen, A36498); Nuclear and Cytoplasmic Extraction Reagents 341 (Thermo, 78835); Zombie VioletTM Fixable Viability Kit (BioLegend, 423113); 342 Pacific Blue anti-mouse CD3 Antibody (BioLegend, 100214); FITC anti-mouse CD8a 343 Antibody (BioLegend, 100706); APC anti-mouse CD8a Antibody (BD Pharmingen, 344 553035); PE/Cyanine7 anti-mouse CD3 Antibody (BioLegend, 100220); APC anti-345 mouse IFN-γ Antibody (Invivogen, 17-7311-82); PE-Labeled Mouse H-346 2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (Acrobiosystems, H2A-MP2H7); 347 Capicua Antibody (Novusbio, NB110-59906); anti-FLAG Antibody (Sigma-Aldrich, 348 F3165); β-actin (Sigma-Aldrich, A2228); Bim Antibody (Cell Signaling Technology, 349 2933T); Bcl-2 Antibody (Cell Signaling Technology, 3498T); Bax Antibody (Cell 350 Signaling Technology, 2772T); Bcl2 antibody (Proteintech, 68103-1-Ig); BAX 351 antibody (Proteintech, 60267-1-Ig); KEAP1 antibody (Proteintech, 10503-2-AP); 352 Anti-mouse Ig HRP (Rockland Immunochemicals, 18-8817-33); Anti-rabbit IgG HRP 353 (Rockland Immunochemicals, 18-8816-33); HRP-conjugated Mouse anti-Rabbit IgG 354 Light Chain (ABclonal, AS061); Prestained Protein Ladder 10-180 kDa (Thermo, 355 26616); Prestained Protein Ladder 25-400 kDa (Sevenbio, SW179). .. 356 4.2 Cells 357 CTLL-2 cells were purchased from the Cell Resource Center, Peking Union Medical 358 College (IBMS, CAMS/PUMC) and cultured in RPMI-1640 (Gibco) supplemented 359 with 10% fetal bovine serum (FBS) and 1× penicillin–streptomycin (Invitrogen), 10 360 mM HEPES, 1 mM Sodium pyruvate (Gibco), 2mM GlutaMAX (Gibco), and 100 361 U/ml IL-2.

    Extraction:

    Article Title: Capicua mediates interleukin-2 withdrawal–induced death of activated CD8+ T cells
    Article Snippet: .. Materials and methods 336 4.1 Reagents and antibodies 337 Reagents and antibodies used in this study were obtained from the indicated sources: 338 Recombinant human IL-2 (SL Pharm, S19991010); Annexin V-FITC/PI Apoptosis Kit 339 (Elabscience, E-CK-A211); Actinomycin D (MCE, HY-17559 ); TrueCut Cas9 340 Protein (Invitrogen, A36498); Nuclear and Cytoplasmic Extraction Reagents 341 (Thermo, 78835); Zombie VioletTM Fixable Viability Kit (BioLegend, 423113); 342 Pacific Blue anti-mouse CD3 Antibody (BioLegend, 100214); FITC anti-mouse CD8a 343 Antibody (BioLegend, 100706); APC anti-mouse CD8a Antibody (BD Pharmingen, 344 553035); PE/Cyanine7 anti-mouse CD3 Antibody (BioLegend, 100220); APC anti-345 mouse IFN-γ Antibody (Invivogen, 17-7311-82); PE-Labeled Mouse H-346 2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (Acrobiosystems, H2A-MP2H7); 347 Capicua Antibody (Novusbio, NB110-59906); anti-FLAG Antibody (Sigma-Aldrich, 348 F3165); β-actin (Sigma-Aldrich, A2228); Bim Antibody (Cell Signaling Technology, 349 2933T); Bcl-2 Antibody (Cell Signaling Technology, 3498T); Bax Antibody (Cell 350 Signaling Technology, 2772T); Bcl2 antibody (Proteintech, 68103-1-Ig); BAX 351 antibody (Proteintech, 60267-1-Ig); KEAP1 antibody (Proteintech, 10503-2-AP); 352 Anti-mouse Ig HRP (Rockland Immunochemicals, 18-8817-33); Anti-rabbit IgG HRP 353 (Rockland Immunochemicals, 18-8816-33); HRP-conjugated Mouse anti-Rabbit IgG 354 Light Chain (ABclonal, AS061); Prestained Protein Ladder 10-180 kDa (Thermo, 355 26616); Prestained Protein Ladder 25-400 kDa (Sevenbio, SW179). .. 356 4.2 Cells 357 CTLL-2 cells were purchased from the Cell Resource Center, Peking Union Medical 358 College (IBMS, CAMS/PUMC) and cultured in RPMI-1640 (Gibco) supplemented 359 with 10% fetal bovine serum (FBS) and 1× penicillin–streptomycin (Invitrogen), 10 360 mM HEPES, 1 mM Sodium pyruvate (Gibco), 2mM GlutaMAX (Gibco), and 100 361 U/ml IL-2.



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    96
    Cell Signaling Technology Inc cell signaling technology 349 2933t
    Cell Signaling Technology 349 2933t, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+signaling+technology+349+2933t/Bim+Rabbit+mAb/10__1016_slash_j__cellin__2026__100323-165-122-122
    Average 96 stars, based on 1 article reviews
    cell signaling technology 349 2933t - by Bioz Stars, 2026-10
    96/100 stars
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